Extended Data Fig. 2: RbCBM74 is a singular globular ___domain, most similar to TmCBM9. | Nature Structural & Molecular Biology

Extended Data Fig. 2: RbCBM74 is a singular globular ___domain, most similar to TmCBM9.

From: The Ruminococcus bromii amylosome protein Sas6 binds single and double helical α-glucan structures in starch

Extended Data Fig. 2

a. Structure of RbCBM74 (PDB 7uww) colored from N-terminus (blue) to C-terminus (red). b. Short β-strands leading into and out of RbCBM74 ___domain are colored in red and blue. c. Overlay of TmCBM9 (gold) (PDB 1i82-A) and RbCBM74 (blue). The DALI server calculated an RMSD of 3.2Å and sequence identity of 17%. d. Close-up view of TmCBM9 binding site showing the two TmCBM9 Trp residues involved in binding cellobiose (gold) and W373 of RbCBM74 (blue) which lies in the same region but is occluded from the surface by a loop containing residues 374-384. e. Zoomed in view of calciums coordinated in the RbCBM74 ___domain with side chains shown in sticks, main chain shown in lines and Ca2+ ions by yellow spheres. Atomic distances are shown in Å and residues are labeled. Residues are colored by element with oxygen shown in red.

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