Extended Data Fig. 6: Cryo-EM analysis of C^NRL4^CSA-E2-Ub-UVSSA and C^NRL4^CSA-E2-Ub complexes.

a. Processing tree for the C^NRL4^CSA-E2-Ub-UVSSA complex. The number of particles included in refined maps is indicated below the map, together with the map resolution. Classes used for further processing are color-coded according to complex subunits. b. Local resolution estimate for the C^NRL4^CSA-E2-Ub-UVSSA map. c. Fourier shell correlation (FSC) plot for the C^NRL4^CSA-E2-Ub-UVSSA map. d. 3D FSC plot for the C^NRL4^CSA-E2-Ub-UVSSA map. e. Processing tree for the C^NRL4^CSA-E2-Ub complex. The number of particles included in refined maps is indicated below the map, together with the map resolution. Classes used for further processing are color-coded according to complex subunits. f. Local resolution estimate for the C^NRL4^CSA-E2-Ub map. g. Fourier shell correlation plot for refined C^NRL4^CSA-E2-Ub map. h. 3D FSC plot for the C^NRL4^CSA-E2-Ub map.