Extended Data Fig. 2: Sequencing and expression of ELOF1 and CSA mutants. | Nature Structural & Molecular Biology

Extended Data Fig. 2: Sequencing and expression of ELOF1 and CSA mutants.

From: Structural basis for RNA polymerase II ubiquitylation and inactivation in transcription-coupled repair

Extended Data Fig. 2

a. Expression of the indicated GFP-ELOF1 proteins (or empty control) in RPE1-iCas9 ELOF1-KO cells. The data shown represent at least three independent experiments. Scale bar, 15 μm. b. Sanger sequencing of genomically integrated cDNA to confirm the presence of mutations. c. Western blot analysis of GFP-ELOF1 proteins (or empty control) in RPE1-iCas9 ELOF1-KO cells. The data shown represent at least three independent experiments. d. Expression of the indicated CSA-GFP proteins (or empty control) in RPE1-iCas9 CSA-KO cells. The data shown represent at least three independent experiments. Scale bar, 15 μm. e. Western blot analysis of CSA-GFP proteins (or empty control) in RPE1-iCas9 CSA-KO cells. The data shown represent at least three independent experiments.

Source data

Back to article page