Extended Data Fig. 3: Effects of Khc tail truncations on motor association with osk RNA and Tm1.
From: Tropomyosin 1-I/C coordinates kinesin-1 and dynein motors during oskar mRNA transport
a, b, Fraction of Khc FL, Khc910, Khc940 and KhcΔAMB microtubule-binding events that co-localized with osk 3′ UTR in absence or presence of Tm1 FL (a) or that localized with Tm1 FL (b). For all plots, mean ± s.d. is shown and is derived from 16–59 microtubules (N) from 4–15 imaging chambers and 2–5 independent experiments (n) per condition (see Supplementary Table 1). Mean values for each independent experiment (large dots) are superimposed on values for individual microtubules (small dots). Black or white circles indicate the presence or absence of indicated components, respectively. Parallel experiments within the figure are shown with large dots of the same color. Cy3-osk 3′ UTR was included in all experiments. Statistical significance was determined by unpaired two-tailed t-tests using N values (total number of microtubules). ns: not significant (p > 0.05).
