Extended Data Fig. 10: Tm1-induced association of Khc with DDBE is dependent on RNA.
From: Tropomyosin 1-I/C coordinates kinesin-1 and dynein motors during oskar mRNA transport
a, Kymographs showing reduced association of Khc FL with motile DDBE-osk 3′ UTR 2 + 3 RNPs in the presence of Tm1 FL after treatment with RNase A. Microtubule plus and minus ends are oriented toward the right and left of each kymograph, respectively. b, Fraction of moving DDBE-osk 3′ UTR 2 + 3 RNPs that colocalized with Khc FL signal with and without RNase A treatment in the presence of Tm1 FL. The mean ± s.d. is shown and is derived from 15–17 microtubules (N), representing analysis of 963–1,079 individual complexes from 4 imaging chambers and 2 independent experiments (n) per condition. Mean values for each independent experiment (large dots) are superimposed on values for individual microtubules (small dots). Black or white circles indicate the presence or absence of indicated components, respectively. Parallel experiments are shown using large dots of the same color. Statistical significance was determined by an unpaired two-tailed t-test with Welch’s correction using N values (total number of microtubules).
