Fig. 4: Tm1 binding affects the free and motor-bound Khc tail.
From: Tropomyosin 1-I/C coordinates kinesin-1 and dynein motors during oskar mRNA transport

a, 1H-15N HSQC spectrum of the Khc tail construct Khc 855–975 with backbone assignments indicated. b,c, 1H-15N HSQC titration experiment with U10 RNA and Tm1 1–335. Overlaid spectra (b) and plotted intensity ratios of individual residues (c). RNA was added before Tm1. d, Intensity ratios of a titration experiment with Tm1 1–335 and U10 RNA in which Tm1 was added before RNA. e, Intensity ratios of a titration experiment with U10 RNA and Tm1 252–334 in which RNA was added before Tm1. f–h, 1H-15N HSQC titration experiment of the Khc tail (Khc 855–975) with the motor ___domain (Khc 1–365) and Tm1 1–335. Overlaid spectra (f), plotted intensity ratios (g) and chemical shift perturbation (CSP) plot (h) of individual residues are presented. Khc 1–365 was added before Tm1 1–335. Data are presented as measured value ± s.d. Error bars represent estimates of propagated measurement errors of the experimental uncertainties in signal amplitudes.