Extended Data Fig. 3: Validation of altered transcriptional changes upon INTS11 depletion by 4SU-seq and TT-seq.
From: Transcription directionality is licensed by Integrator at active human promoters
a-b. IGV tracks of RNU11, RNU12, and TERC loci from TT-seq (a) and 4sU -seq (b) results before and after INTS11 depletion. c. Heatmap representing the identified 2121 active genes and PROMPTs from GSE223265 TT-seq results (left) and the overlaying heatmap of +IAA/CTRL (right). d. IGV tracks of 4sU-seq representing PROMPT accumulation at MYC or BMP4 loci. e-f. Heatmap (e) and Metagene analysis (f) showing accumulated transcripts in the PROMPT direction and reduced transcripts in the pre-mRNAs direction for 2121 active genes upon INTS11 depletion. g. Quantitative boxplots of transcription distribution at PROMPTs (left) and pre-mRNA (right) from 4sU-seq results. P values were calculated by two-sided Mann-Whitney test. Boxplots were plotted as before. h-i. The IGV track comparison of 4sU-seq (h) and TT-seq (i) at the XPO1 or EP300 loci upon INTS11 depletion.
