Extended Data Fig. 1: Expression and purification of SID-1 variants.
From: Structural insights into double-stranded RNA recognition and transport by SID-1
a-c, Expression levels of SID-1 variants in insect S2 cells by western blot. The S2 cells were prepared under the same conditions to the cells used for luciferase activity assay. n = 3 independent experiments. d-f, The representative gel filtration profiles of purified SID-1, hSIDT1, and SIDT2 for cryo-EM anaylsis. n = 3 independent experiments. g-k, Purificaiton of SID-13A (g), SID-15E (h), SID-1D70A/D171A (i), SID-1R172W/A173T (j), and SID-1L353-S423 (k) for dsRNA MST binding assay. n = 3 independent experiments. l, Cellular localization of SID-1 and SID-1ΔL353-S423 by confocal imaging. Cell membrane was stained by Dil (red). A GFP was fused to SID-1 and SID-1ΔL353-S423 for imaging, respectively. n = 3 independent experiments. Scale bars, 10 μm.
