Extended Data Fig. 5: Further characterization of Tet1/2 DKO ESCs’ response to mTORi-induced dormancy.
From: TET activity safeguards pluripotency throughout embryonic dormancy
a. Correlation of pathway expression in wild-type and Tet1/2 DKO ESCs at 24 h and at 72 h of mTORi treatment. Significance of correlation was tested by a linear regression t-test (two-tailed). b. Differentially expressed genes in Tet1/2 DKO vs. wild-type at 72 h of mTORi treatment. P-values and log2 fold changes were derived using DEseq2. c. DNA methylation levels of different genomic features in wild-type vs Tet1/2 DKO ESCs. White dots show the median; vertical box plots within violin plots show interquartile range (IQR) and the whiskers show 1.5 IQR. d. RNA expression levels of Tet and Dnmt enzymes in untreated vs mTORi treated wild-type ESCs over time. TPM, transcripts per kilobase million. e. Expression of TET1 and TET2 proteins in untreated vs mTORi treated (144 h) wild-type ESCs. N: nuclear, C: cytoplasmic extract. Three biological replicates were performed.
