Extended Data Fig. 7: Further analysis of TET-RNA binding.
From: TET activity safeguards pluripotency throughout embryonic dormancy
a. Schematics of FLASH tag insertion into the Tet1 and Tet2 loci. b. Western blots showing successful pull-down of the tagged TET1 and TET2 proteins. c. Differential binding levels of TET1 to repeat RNAs at 24 h and 144 h compared to 0 h. Gray indicates no significant differences between the time points. P-values and log2 fold changes were derived using DEseq2. d. Expression levels of top genes that contact TET-target active enhancers (ABC-score49>0.02) as determined by analysis of published HiC and H3K27ac datasets. TET activity at enhancers is largely uncoupled from transcription under dormancy conditions.
