Extended Data Fig. 8: Further investigation of chromatin dynamics of pausing.
From: TET activity safeguards pluripotency throughout embryonic dormancy
a. Levels of the indicated histone marks at TET-target primed enhancers, the Other category (see Fig. 4c), and at all TET-bound peaks in wild-type and Tet1/2 DKO cells over 144 h time course of mTORi-mediated pausing and release. Tet1/2 DKO cells were paused for 72 h to avoid loss of pluripotent colonies. Lines show mean values, shade shows the confidence interval. Dashed lines denote levels of each mark at 0 h in each genetic background. b. Levels of the indicated histone marks at the shown regions during longer-term pausing. Time points up to 15 days were collected in wild-type cells. Lines show mean values, shade shows the confidence interval.
