Extended Data Fig. 6: Mutation of psd and pus7 is synthetically lethal in Arabidopsis.
From: Pseudouridine guides germline small RNA transport and epigenetic inheritance
(a) Alexander staining of pollen from Col-0, pus7, psd-13, psd-13−/−; pus7 + /−, and psd-13 + /−; pus7−/− parents. psd-13 + /; pus7−/− had semisterile pollen abortion (loss of Alexander staining, arrows). (b) Mature seeds from each parent. Aborted seeds in psd-13are highlighted with arrows. (c) Quantification of approximately 500 seeds from each genotype revealed psd-13−/−; pus7 + /− aborts seeds at a rate of ~25% (* indicates no difference from expected 3:1 normal:aborted ratio, p > 0.1 Chi-square test). Double homozygous mutants could not be obtained (n = 150 plants).
