Extended Data Fig. 4: Mapping of miRNA pseudouridylation sites in Arabidopsis by CMC labeling and reverse transcription in the presence of Mn2+ (CMC/Mn2+).
From: Pseudouridine guides germline small RNA transport and epigenetic inheritance
(a, b) Bar chart showing proportions of 5ʹ and 3ʹ nucleotides in Ψ-IP-enriched (a) and CMC-depleted (b) siRNAs from inflorescence compared to background (non-structural) small RNAs. IP-enrichment and CMC-depletion show biases towards 5ʹ and 3ʹ U. (c) Sites of predicted Ψ (insertions and deletions) in precursor pri-miR159b PCR amplicons (upper panels) and mature miR159b (lower panel; high-throughput sequencing) in seedlings, leaves and flower buds of wild-type and hyl1-2 microprocessor mutants. pri-miRNA amplicons (upper) and mature small RNA sequences (lower) had insertions and deletions at predicted sites. Number of clones with the respective amplicon is shown in brackets.
