Extended Data Fig. 7: Biochemical characterisation of the FHF and KIF1C stalk interaction.
From: KIF1C activates and extends dynein movement through the FHF cargo adapter
a, Size Exclusion Chromatography (SEC) of isolated full-length FHF and FHF + KIF1C stalk (residues 642-922). Experiment repeated three times. b, Mass photometry of full-length FHF and KIF1C stalk using the Refeyn machine. Cartoon on the right depicts the KIF1C stalk construct used for a-b. Experiment performed once. c, SEC reconstitution of FH451-718F in the absence or presence of two shorter KIF1C stalk constructs (residues 674-922 and 674-822, respectively) depicted in the cartoons on the right. Experiment repeated twice. d, SEC reconstitution of full-length FHF in the presence and absence of the KIF1C stalk SGD ___domain. Two SGD deletion constructs within KIF1C stalk were tested (∆722-840 and ∆742-840) against SGD construct (residues 722-841), as depicted in the cartoons on the right. Experiment performed once. e, SEC reconstitution of truncated FH629-718F with SGD construct shows no binding. Experiment performed once. SDS-PAGE gels for all runs show fractions from the peak regions.
