Extended Data Fig. 9: Subclassification of motor complex motility and pausing events.
From: KIF1C activates and extends dynein movement through the FHF cargo adapter
a, Example kymograph and annotation showing what would be classified as plus and minus end directed motility and paused (moving < 25 nm/s). The example shows the TMR-dynein channel of a DDHK chamber. The scale bar is 20 μm and 20 s in the horizontal and vertical axes, respectively. b-d, Superplots of HKFL, DDH and DDHKFL time spent moving towards the plus end (b), time spent moving towards the minus end (c) and time spent pausing at a speed < 25 nm/s (d). Note that wholly static tracks (total distance <1000 nm) are excluded from this analysis. Small dots show data per microtubule and large dots show experimental averages. Boxes show quartiles with whiskers spanning 10%-90% of the data. n=94, 165 and 202 for HKFL, DDH and DDHKFL, respectively. Exact p values shown above graphs using Kruskal Wallis H test used followed by a Conover’s posthoc test to evaluate pairwise interactions with a multiple comparison correction applied using Holm–Bonferroni Source data.
