Table 1 Cryo-EM data collection, refinement and validation statistics
THIK-1 (EMDB-50741), (PDB 9FT7) | |
|---|---|
Data collection and processing | |
Magnification | ×105,000 |
Voltage (kV) | 300 |
Electron exposure (e–/Å2) | 42.54 |
Defocus range (μm) | −1 to −2.3 |
Pixel size (Å) | 0.832 |
Symmetry imposed | C2 |
Initial particle images (no.) | 9,257,778 |
Final particle images (no.) | 302,189 |
Map resolution (Å) | 3.53 |
FSC threshold | 0.5 |
Map resolution (Å) | 3.16 |
FSC threshold | 0.143 |
Map resolution range (Å) | 2.72–8.56 |
Refinement | |
Initial model used (PDB code) | De novo |
Model resolution (Å) | 3.29 |
FSC threshold | 0.5 |
Model resolution range (Å) | 2.72–8.56 |
Map sharpening B factor (Å2) | −140 |
Model composition | |
Non-hydrogen atoms | 4,016 |
Protein residues | 508 |
Potassium ions | 2 |
Linoleic acid | 2 |
B factors (Å2) | |
Protein | 64.73 |
Potassium ion | 23.63 |
Linoleic acid | 48.43 |
R.m.s. deviations | |
Bond lengths (Å) | 0.007 |
Bond angles (°) | 1.064 |
Validation | |
MolProbity score | 0.85 |
Clashscore | 1.26 |
Poor rotamers (%) | 0 |
Ramachandran plot | |
Favored (%) | 98.82 |
Allowed (%) | 1.18 |
Disallowed (%) | 0 |
