Extended Data Fig. 8: In vitro validation of the IpaH1.4–RNF213 binding interface.
From: Shigella flexneri evades LPS ubiquitylation through IpaH1.4-mediated degradation of RNF213
(a) Pulldown of the indicated Flag-GFP-tagged RNF213 proteins from cell lysates upon incubation with purified GST-IpaH1.4 (or GST as negative control) as bait. Mutated RNF213 residues and their interaction partners in IpaH1.4 are highlighted in the cartoon. (b) Pulldown of Flag-GFP-RNF213 from cell lysate upon incubation with GST or GST-tagged IpaH (wild type or mutant) as bait. Mutated residues in IpaH1.4 and their interaction partners in RNF213 are highlighted in the cartoon. The results in both panels are representative of n = 3 experiments each.
