Extended Data Fig. 8: Forward genetic screening identified TOFU-3 as required for UAD-2 condensate formation and piRNA production.
From: piRNA gene density and SUMOylation organize piRNA transcriptional condensate formation
a. Schematic representation summarizing piRNA genes and alleles identified from the two forward genetic screens. b. Images of the meiotic region of the indicated adult animal germline grown at 20 °C. All images were taken by the Leica THUNDER imaging System and deconvoluted using Leica Application Suite X software (version 3.7.4.23463). All images are representative of more than three animals (see also in Extended Data Fig. 8c, d and f). c, Complementation test of two alleles of tofu-3 at 20 °C. d, Images of pachytene cells of the indicated adult animals grown at 20 °C. mCherry::TOFU-3 rescued UAD-2::GFP foci formation in tofu-3(tm3068) cells. e, Sequence alignment of the TOFU-3 (ULP-5) protein. f, Subcellular localization of UAD-2::GFP in pachytene cells in eri-1(mg366);UAD-2::GFP worms fed the indicated RNAi bacteria. n, the number of independent animals tested. Source data are provided as a Source Data file. g, Deep sequencing of total small RNAs of the indicated adult animals from one biological replicate. Blue dashed lines indicate piRNAs. h, Scatter plots depicting the expression levels of Cluster I, Cluster II, and out-Cluster piRNAs in the wild-type (x-axis) and tofu-3 mutant strains (y-axis) from one biological replicate, normalized as reads per million+1.
