Extended Data Fig. 2: 2D lysozyme film prepared with cysteine. | Nature Protocols

Extended Data Fig. 2: 2D lysozyme film prepared with cysteine.

From: Synthesis and functionalization of scalable and versatile 2D protein films via amyloid-like aggregation

Extended Data Fig. 2

a, The NPM assay for native lysozyme and partially unfolded lysozyme. The inset shows the reaction between NPM and free thiol to enhance the fluorescence emission. b, Typical MALDI-TOF-MS spectra of native lysozyme and cysteine-reduced lysozyme. c, The SDS-PAGE of native lysozyme (lane 1) and partially unfolded lysozyme (lane 2). d,The activity assay of native lysozyme, cysteine and partially unfolded lysozyme. e, ANS assay for native lysozyme, cysteine, and partially unfolded lysozyme. f, Intrinsic tryptophan fluorescence assay for native lysozyme and partially unfolded lysozyme. Figure adapted with permission from ref. 23, Wiley.

Source data

Back to article page