Extended Data Fig. 4: Characterization of the 2D lysozyme film prepared with cysteine.

a, Digital photograph of a large-area protein film at the air/water interface. b,c, AFM (b) and TEM (c) images of the protein film surface. d, UV/vis absorption spectrum of the protein film in the range of 200-800 nm, along with a digital photograph showing the film-coated quartz with a leaf as a background. e, Water contact angle of the protein film coated on different substrates. f, CD spectra of the protein film stained with ThT. g, Digital photograph of the protein film dyed by Congo Red and an CLSM image of the ThT-dyed protein film. h, The thickness of the film as a function of lysozyme concentration. i, The effect of repetitive coating times on the thickness of the nanofilm. Figure adapted with permission from ref. 23, Wiley.