Extended Data Fig. 3: BEHAV3D imaging on SP8. | Nature Protocols

Extended Data Fig. 3: BEHAV3D imaging on SP8.

From: BEHAV3D: a 3D live imaging platform for comprehensive analysis of engineered T cell behavior and tumor response

Extended Data Fig. 3

a, Representative 3D multispectral images of ALL tumor cells (yellow) in co-culture with CD8+ (blue) and CD4+ (green) CAR19 cells, showing tumor response (red) at the indicated time points. Scale bars, 100 µm. b and c, Single channels showing raw images (b) and corrected images after Step 37 (c) for CD8+ CAR19 (top) and CD4 CAR19 (bottom) channels.

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