Poorly performing antibodies contribute to the reproducibility crisis in biomedical research. The market offers millions of antibodies and validating them is a cumbersome process. Now, the non-profit Antibody Characterization through Open Science (YCharOS) organization shares a step-by-step protocol for rigorous antibody validation using genetic (knockout) cell line controls.
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Acknowledgements
We thank M. Uhlén (KTH, the Human Protein Atlas and Antibodypedia) for discussions.
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J.L.A.V. is CEO of the company Aeonian Biotech. F.L.M. and C.W. declare no competing interests.
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Monteiro, F.L., Voskuil, J.L.A. & Williams, C. YCharOS protocol for antibody validation. Nat Protoc 20, 1389–1390 (2025). https://doi.org/10.1038/s41596-024-01108-6
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DOI: https://doi.org/10.1038/s41596-024-01108-6
