Correction to: Nature Protocols https://doi.org/10.1038/s41596-023-00930-8, published online 19 January 2024

In the version of this article initially published, there were mistakes in the timing listed in the abstract, where in the text now reading “Constructing the antigen and control tetramers takes 12 h,” “9 h” appeared originally; in the Fig. 1 overview, where Steps 41–53 should take 4 h (not 1 h); subhead above Step 41, where in the text now reading “Producing control tetramer (4 h, add 6 h for biotinylating control antigen)” “4 h” appeared originally as “1 h”; in the Timing section, where Part 1 now appears as “12 h,” not “9 h,” and for Steps 41–53 in Timing where “4 h” now replaces “1 h.” For reader clarity, the final column heading in the Step 18 table has been updated to “SA concentration” from “Final concentration”; and in the Step 20 table, row 2 under “Volume of SA–PE (Steps 17–18)” has been updated to “5 μL of PBS” from “0”, row 9 under “Volume of 2 μM biotinylated antigen (Step 19)” has been updated to “5 µL of PBS” from “0,” and in row 10, the entry under “Volume of 2 μM biotinylated antigen (Step 19)” has been updated to “10 µL of a positive control biotinylated antigen” from “10 μL” and the entry under “Total amount of antigen per tube (pmol)” has been updated to “20 pmol” from “20 pmol of a biotinylated control antigen.” Steps 5 and 37 have been amended to describe what to do with the retentate; the calculation in Step 8 has been updated from the original “volume of biotin (µL) = (antigen concentration (µM) × antigen volume (µL) / biotin ratio) × biotin concentration (µM)” and the second Critical Step in Step 8 is updated from the original text “To avoid low pipetting volumes, biotin can be diluted to 0.1–1 mM and this must be considered in the calculation in this step.” The second sentence of Step 32 has been amended to read “Use the SA amount (table in Step 20) in the lane where the band reappears...” from the original “Use the concentration in the lane where the band reappears...”. The first calculation in Step 32 has been updated from the original “SA concentration where band emerges (μM) × 4 biotin binding sites × dilution factor (from step 19) = biotinylated antigen concentration (μM).” In the Step 50 table, in the extinction coefficient for APC-DL755, “0.7 μM” has replaced “0.6 μM.” All changes have been made in the HTML and PDF versions of the article.