Fig. 3

Correlation of mRNAsi with TIME different patterns of TNBC patients, different functional enrichment analyses, and survival outcomes between mRNAsi groups. (a) Based on the ssGSEA immune signature the mRNAsi of TNBC patients were categorized into two subgroups low and high group based. Red color indicates a high mRNAsi group and green color indicates a low mRNAsi group, the expression of an estimate, stromal, and immune, mRNAsi groups in the immune signature were indicated by red and blue color, red shows upregulation, and blue shows downregulation (b–d) Correlation between mRNAsi, the immune score, stromal score, and ESTIMATE score based on ESTIMATE algorithm. The blue line is the regression line of mRNAsi and other scores. (e) Comparisons of the abundances of 22 immune cells in 2 mRNAsi groups by CIBERSORT algorithm. *p < 0.05; **p < 0.01; ***p < 0.001, ****p < 0.0001. (f) mRNAsi were divided into two groups, high and low based on survminer analysis determined 0.47 as the optimal grouping, with value of high mRNAsi group (N = 85) and low mRNAsi group (N = 42). (g) The heatmap reflects the expression levels of DEGs between high and low mRNAsi groups. (h-i) The GO and KEGG functional annotation analysis of the DEGs. (j) KM survival analysis showed the different survival status between mRNAsi groups green color shows low mRNAsi and the red color shows high mRNAsi group with a p-value of 0.041. Significance P values were calculated by the Wilcoxon rank sum test.