Fig. 5

The Sashimi plot of DAS gene MSTRG.32525 undergo differential alternative splicing under biotic stressors. Sashimi plots depict differential alternative splicing (DAS) through alternative 5′ splice site (A5SS) events of gene MSTRG.32525 in response to different biotic stressors. Individual plots visualize the read coverage and junction reads which are plotted as arcs with the indicated junction read counts. Below each plot alternative splicing isoforms are visualized in dark blue. (a) Control (Xag8ra_mock, gray) and AS in response to Xanthomonas axonopodis pv. glycine 8ra (Xag8ra, green), (b) control (P. capsici_mock, gray) and AS in response to Phytophthora capsici (P. capsici, blue), (c) control (P. infestans_mock, gray) and AS in response to Phytophthora infestans (P. infestans, orange). The Benjamini–Hochberg method was applied to obtain p-values corrected for false-discovery rate (FDR), and DAS events were identified using a cutoff of |ΔIncLevel| ≥ 0.1 and FDR < 0.05.