Fig. 1
From: Tandem mass spectral metabolic profiling of 54 actinobacterial strains and their 459 mutants
Overview of LC-MS/MS dataset describing the metabolic profiling of the 54 actinobacterial strains and their 459 mutants. (a) The experimental workflow involves the following steps: we start from (1) an actinobacterial collection of 54 wild type strains, which are firstly (2) genetically activated by integrating overexpression cassettes of 5 global regulators (Crp, AdpA, RedD, FAS, SarA), (3) followed by OSMAC fermentation of these strains and their mutants in 3–5 different media to generate 2,138 samples. (4) Subsequently, sample preparation is carried out through lyophilization and solvent extraction, (5) followed by LC-MS/MS analysis of the fermentation extracts and (6) overall molecular networking data analysis. (b) Phylogenetic tree of 16S rRNA sequences of 50 strains14 and 4 model Streptomyces (Streptomyces venezuelae, Streptomyces griseus, Streptomyces coelicolor and Streptomyces albidoflavus).
