Fig. 1 | Scientific Data

Fig. 1

From: Coding and non-coding RNA expression in NSC34 cells following TDP-43 depletion and mutant TDP-43 M337V expression

Fig. 1

RNA-Seq workflow and analysis pipeline. (a) Steps for RNA-seq library preparation, including RNA extraction, quality checks, and library construction for both experimental settings: TDP-43 Knockdown (KD) with its respective control (NS), and TDP-43 mutant expression (MVT) with its respective control (WTT). (b) Bioinformatics pipeline for processing raw FASTQ data, from quality trimming to gene expression quantification, including the software tools used. (c) Workflow for read normalization, sample quality control, and gene biotype annotation as reported in this manuscript.

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