Fig. 3
Overview of sequencing quality control. The left plots correspond to the KD vs. NS experiment, while the right plots correspond to the MVT vs. WTT experiment. (a) Per-base sequence quality scores demonstrate consistently high read-quality across the entire read length for all samples in both experiments, with most bases having Phred scores ≥ 30. (b) Per-sequence Phred quality scores show that the majority of reads exhibit high sequence quality, with Phred scores predominantly above 30, ensuring reliable base calls across all samples. (c) Sequence count distributions illustrate the estimated proportions of unique (blue) and duplicate (black) reads in each sample. The majority of reads are unique, confirming high sequencing depth and library complexity suitable for downstream differential expression analyses.
