Figure 6

Hyaluronidase injection significantly alters splenocyte T cell populations. Analysis of cytokine expression and Th cell population analysis in total mouse spleen tissue. (A) ELISA analysis of the levels of TGF-β, IL-4, IFN-γ on spleen. Data are expressed as median optical intensities. Data are presented as means ± SEMs. (B) Representative flow cytometry results of TH1 and TH2 cell populations. Data are presented as means ± SEMs. (C,D) Quantitative real-time PCR analysis of IL-6 and HABP2 mRNA expression. Data are presented as means ± SEMs. *P < 0.05, **P < 0.005, ***P < 0.001.