Figure 4 | Scientific Reports

Figure 4

From: Reduced biological effect of e-cigarette aerosol compared to cigarette smoke evaluated in vitro using normalized nicotine dose and RNA-seq-based toxicogenomics

Figure 4

Volcano plots for the 9 RNA-seq contrasts. On top, air vs 3R4F 1/30 smoke dilution at 24 hrs (A), 48 hrs (B) post exposure, and air vs 3R4F 1/30 smoke adjusted for post exposure time (C). In the middle, air vs e-cigarette aerosol 1/7 dilution at 24 hrs (D), 48 hrs (E) post exposure, and air vs e-cigarette aerosol 1/7 dilution adjusted for post exposure time (F). At the bottom, air vs e-cigarette aerosol 1/3 dilution at 24 hrs (G), 48 hrs (H) post exposure, and air vs e-cigarette aerosol 1/3 dilution adjusted for post exposure time (I). The X-axis shows the Log2 of the fold change (FC) and the Y-axis shows the −Log10 of the adjusted p-values (pFDR). The dotted horizontal lines show the 0.05 pFDR threshold (blue line) and the 0.01 pFDR threshold (red line). The vertical red lines show the +1.5 and −1.5 fold change thresholds. Blue dots represent significant RNA features at pFDR < 0.05 and −1.5 > FC > +1.5 and red dots represent significant RNA features at pFDR < 0.01 and −1.5 > FC > +1.5.

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