Figure 7 | Scientific Reports

Figure 7

From: Reduced biological effect of e-cigarette aerosol compared to cigarette smoke evaluated in vitro using normalized nicotine dose and RNA-seq-based toxicogenomics

Figure 7

qPCR validation of RNA-seq data in MucilAir™ cells from 3 different subjects at 24 hrs post-exposure. Sets of 20 genes per treatment were selected from the RNA-seq data for qPCR screening in MucilAir™. PCA plots of qPCR data comparing treatments (air vs 3R4F 1/30 (A), e-cigarette 1/7 (B) and e-cigarette 1/3 (C)) and subjects (#MD058501, #MD008301, #MD009101). Corresponding volcano plots showing the response for each individual gene using the data from the three donors (3R4F 1/30 (D), e-cigarette 1/7 (E) and 1/3 (F)). Dots colored in red indicate differentially expressed genes significant at p < 0.05 and with a FC > [1.5]. The red dots are labelled with the gene name.

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