Figure 2
From: Rational modular design of metabolic network for efficient production of plant polyphenol pinosylvin
Rational modular design of the overall pathway to remove the pathway bottleneck. (A) A series of module one and two expression cassettes were designed at different expression levels. (B) The concentrations of pinosylvin were achieved by different expression cassettes (S1–S8). (C) Different pinosylvin concentrations were obtained by expression cassettes between S9–S20. (D) Different cinnamic acid concentrations were obtained by expression cassettes between S9–S20. COLA: origin of pCOLADuet-1; CDF: origin of pCDFDuet-1; pBR322: origin of pETDuet-1; p15A: origin of pACYCDuet-1; RSF: pRSFDuet-1; Trc: Trc promoter; T7: T7 promoter. S1–S20 demotes strains 1–20 constructed in the study.
