Figure 5
From: Network and role analysis of autophagy in Phytophthora sojae
Induction of autophagy in response to rapamycin treatment in P. sojae. (A) Autophagy indicated by GFP-PsAtg8. GFP-PsAtg8-expressing P. sojae hyphae were incubated in 10% V8 juice. After treated with 100 nM rapamycin or DMSO (control) for 4 h, hyphae were analyzed by confocal microscopy. Bars = 10 μm. (B) Autophagy indicated by MDC dye. P. sojae wild-type hyphae were incubated in 10% V8 juice. After treated with 100 nM rapamycin or DMSO (control) for 4 h, hyphae samples concurrently stained with MDC were analyzed by confocal microscopy. Bars = 10 μm.
