Figure 6

P. sojae sporangial production is regulated by autophagy. (A) Visualization of autophagic activation with MDC in P. soaje mycelia (MY) and zoosporangia (SP). Wild-type hyphae were incubated in 10% V8 juice for 30 h. After 3 washes and incubation with sterile distilled water for 4 h, hyphae were concurrently stained for MDC and analyzed by confocal microscopy. Bars = 10 μm. (B) Numbers of sporangia produced by PsAtg6a transgenic lines. Sporangia of the indicated samples were counted 12 h after the induction of sporangial production. Representative data are shown from three separate experiments. The bars indicate standard errors, and stars above bars indicate that the difference from the wild-type value was significant. **P < 0.01 (t-test). (C) Sporangial phenotypes forPsAtg6a transgenic lines. Micrographs were taken 12 h after sporangial induction. Black arrows indicate sporangia. Bars = 500 μm.