Figure 4 | Scientific Reports

Figure 4

From: Arginine-rich cell-penetrating peptide-modified extracellular vesicles for active macropinocytosis induction and efficient intracellular delivery

Figure 4

Increased anti-cancer activity of saporin encapsulated in EVs modified by Rn-EMCS. (a) Schematic representation of the encapsulation of saporin (SAP) (PDB (Protein Data Bank) accession number: 1QI7)25 in EVs by electroporation. (b) CHO-K1 cells were treated with SAP encapsulated in EVs (20 μg/ml) (EVs (25 μg) and SAP (50 μg) in electroporation condition as described in Methods section) with or without modification by Rn-EMCS (n = 4, 8, 12: 20 μM, n = 16: 10 μM) for 48 h at 37 °C. Cell viability was then analysed using a WST-1 assay. The data are expressed as the average (±SD) of four experiments. ***p < 0.001. (c) Microscope observations of CHO-K1 cells treated with EV samples under the same experimental conditions as (b).

Back to article page