Figure 3 | Scientific Reports

Figure 3

From: FK866 attenuates acute hepatic failure through c-jun-N-terminal kinase (JNK)-dependent autophagy

Figure 3

Autophagy induction by rapamycin attenuates ALF caused by GaIN/LPS or ConA in mice. Mice were pretreated with rapamycin (Rap, 2 mg/kg, 30 min, IP) prior to GaIN/LPS (G/L, 600 mg/kg /0.5 μg/kg, 7 h, IP) or ConA (20 mg/kg, 6 h, IV) challenge. (a) Determination of ATG7, p62, and LC3B expression in the presence or absence of Rap. The data are shown as the means ± SEM. n = 4–6 per group. *P < 0.05 indicates significant differences. (b) Quantification of serum aminotransferase levels. The data are shown as the means ± SEM. n = 6 per group. *P < 0.05 vs. the corresponding vehicle (Veh) group. (c) H&E staining of mice livers. The arrows denote hepatocellular necrosis; the arrowheads denote infiltrating inflammatory cells. Original magnification × 400, scale bar 50 µm. n = 4 per group. (d) Pretreatment with Rap increased survival rate of mice subjected to G/L or ConA challenge. n = 10–11 per group. *P < 0.05 compared to the Rap-treated group.

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