Figure 4
Histone deactylase inhibition induces histone H3K9 acetylation and HLA class-I expression and does not reduce LT expression in vivo. NOD.CB17/Prkdc scid mice (n = 5 for each treatment group) bearing xenotransplanted WaGa tumors were treated with the drug carrier polyethylene glycol (control, grey), or the combination of vorinostat and mithramycin A (V + MA, dark blue) as described in materials and methods. (A) mRNA was isolated from cryopreserved tumors and RT-qPCR was performed using primers for HLA-A, B2M, TAP1, TAP2, LMP2 and LMP7. CT values were normalized to RPLP0 and calibrated to in vitro cultured WaGa cells. (B) Immunohistochemistry was performed on sections of FFPE fixed tumors using antibodies specific against AcH3K9, LT (clone CM2B4) and HLA-A. A representative example for each group is depicted. Scale bars represent 100 µm.
