Figure 7 | Scientific Reports

Figure 7

From: Comparative genomics and transcriptomics of Escherichia coli isolates carrying virulence factors of both enteropathogenic and enterotoxigenic E. coli

Figure 7

Functional characterization of T3SS in the EPEC/ETEC hybrid isolates. Immunoblot analysis for EspB in the supernatants of traditional EPEC (E2348/69 and 402290), ETEC (H10407), and the EPEC/ETEC hybrid isolates (102651, 102712, 102771, and 401140) grown in DMEM with high glucose (A) or with low glucose (B). Included as a negative control is E. coli CVD452, which is an isogenic T3SS-deficient ∆escN mutant of E2348/6949 and ETEC (H10407) which lacks a T3SS28. The first lane of each image contains the Chameleon Duo pre-stained protein ladder (Li-Cor). Under both conditions all of the EPEC/ETEC isolates have a functional T3SS as determined by the EspB secreted protein. The EPEC/ETEC isolates are indicated in bold.

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