Figure 2

Study of the biological and functional characteristics of RAD51-AS1 in vitro. (A), Relative RAD51-AS1 expression in EOC cell lines. (B), a,b: QRT-PCR and proliferation assay to quantify the silencing efficiencies of RAD51-AS1 in SKOV3 cells transfected with ASO1, ASO2, ASO3 and ASO-mix. c: QRT-PCR confirmed that RAD51-AS1 was efficiently silenced by transfection with ASO-mix in SKOV3, SKOV3.ip and HO8910 cells. (C), The efficiency of RAD51-AS1 overexpression was confirmed by qRT‑PCR (D), Proliferation assays. Cell growth curves were automatically recorded in real-time. Silencing of RAD51-AS1 inhibited cell proliferation in SKOV3, SKOV3.ip and HO8910 cells. Overexpression of RAD51-AS1 increased the proliferation of Hey and OVCAR3 cells. The light-colored error bars denote the SD. (E), Cell cycle assays. RAD51-AS1 knockdown increased the percentage of cells in G1/G0 phase, while the effects on S and G2/M phages are not consistent among the 3 cell lines. (F), Apoptosis assays. RAD51-AS1 knockdown increased the percentage of apoptotic cells in SKOV3, SKOV3.ip and HO8910 cells. Each experiment was repeated in triplicate (n = 3). KD, knockdown of RAD51-AS1 expression. OE, overexpression of RAD51-AS1, NC, negative control. (The results are shown as the mean ± SD; *P < 0.05; **P < 0.01; ***P < 0.001 by Student’s t test).