Figure 4 | Scientific Reports

Figure 4

From: Suppression of VAMP2 Alters Morphology of the Tegument and Affects Glucose uptake, Development and Reproduction of Schistosoma japonicum

Figure 4

Transmission electron microscopy analysis of schistosomes after siRNA treatment. (A,B,C) Topography of S. japonicum in the control group, showing the whole tegument from the surface to the muscle layer. (A) The dorsal aspect of the female schistosome. (B,C) The dorsal aspect of the male schistosome. Tegument matrix (T), cytoplasmic bridge (C), nucleus of syncytium (N), spines (S), muscles (M) beneath the tegument, discoid bodies (Db), membranous bodies (Mb),vesicles (V) in the tegument matrix. (D,E,F) Topography of the tegument of S. japonicum after treatment with SjVAMP2 specific siRNA. (D) Emergence of enlarged membranous bodies with hepatalmemebrane in the tegument matrix of male worm (arrow indicated). (E) Male schistosome showing an irregular enlargement of sensory organelles in the tegument (large arrow), formation of quantities of vacuoles (small arrow). (F) Female schistosomes with decreased thickness of the dorsal medial tegument showing the failure to close the outer tegument invaginations (arrow indicated). (G) Three male worms were randomly selected and the areas occupied by vacuoles were measured by imageJ. (H) Three female worms were randomly selected and the thickness of the dorsal medial tegument was analyzed by imageJ.

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