Figure 5 | Scientific Reports

Figure 5

From: Hypoxia-inducible factor-1 alpha is involved in RIP-induced necroptosis caused by in vitro and in vivo ischemic brain injury

Figure 5

RIP3 siRNA alleviated cell death and decreased the protein level of HIF-1α post OGD in HT-22 cells. (a) The viability of transfected HT-22 cells was detected by MTT assay after 9 h of reoxygenation. (b) The alterations of cell death with different siRNA transfections were analyzed by flow cytometry. (c) The protein expressions of HIF-1α, RIP3, MLKL and phosphorylated MLKL were determined by Western Blotting assay, normalized to β-actin levels. Control siRNA represented nontargeting siRNA transfection and pho-MLKL was the abbreviation of the phosphorylated MLKL. **p < 0.01 vs. Control siRNA group. Also see Supplementary Figure S4.

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