Table 3 Performance of the MLMA assay compared with the conventional method.
MLMA assay | Conventional method | Sensitivity | Specificity | Agreement | Kappa Value | |
|---|---|---|---|---|---|---|
Positive | Negative | (%) | (%) | (%) | ||
Non-O1/O139 V. cholerae | ||||||
 Positive | 17 | 0 | 100.0 | 100.0 | 100.0 | 1.00 |
 Negative | 0 | 252 |  |  |  |  |
O1 V. cholerae | ||||||
 Positive | 43 | 0 | 100.0 | 100.0 | 100.0 | 1.00 |
 Negative | 0 | 226 |  |  |  |  |
O139 V. cholerae | ||||||
 Positive | 2 | 0 | 100.0 | 100.0 | 100.0 | 1.00 |
 Negative | 0 | 267 |  |  |  |  |
V. fluvialis | ||||||
 Positive | 14 | 1 | 93.3 | 100.0 | 99.6 | 0.96 |
 Negative | 0 | 254 |  |  |  |  |
V. vulnificus | ||||||
 Positive | 20 | 0 | 100.0 | 100.0 | 100.0 | 1.00 |
 Negative | 0 | 249 |  |  |  |  |
V. parahaemolyticus | ||||||
 Positive | 139 | 6 | 95.8 | 100.0 | 97.7 | 0.95 |
 Negative | 0 | 124 |  |  |  |  |
V. mimicus | ||||||
 Positive | 1 | 0 | 100.0 | 100.0 | 100.0 | 1.00 |
 Negative | 0 | 268 |  |  |  |  |
V. alginolyticus | ||||||
 Positive | 66 | 0 | 100.0 | 100.0 | 100.0 | 1.00 |
 Negative | 0 | 203 |  |  |  |  |
P. shigelloides | ||||||
 Positive | 4 | 0 | 100.0 | 100.0 | 100.0 | 1.00 |
 Negative | 0 | 245 |  |  |  |  |
Aeromonas | ||||||
 Positive | 11 | 0 | 100.0 | 100.0 | 100.0 | 1.00 |
 Negative | 0 | 258 |  |  |  |  |
