Figure 4
From: GP73 regulates Hepatic Steatosis by enhancing SCAP-SREBPs interaction
GP73 promotes SCAP Trafficking to the Golgi and increases SCAP-SREBP association. (a) Immunoprecipitation analysis of SCAP-SREBP1 association in Flag-vector or Flag-SCAP HepG2 cells transfected with Myc-vector or Myc-GP73. (b) Representative confocal immunofluorescence images of SCAP localized in Golgi apparatus in Ctrl-1, Ctrl-2, GP73-1, and GP73-2 cells. SCAP, red; GM130, green. DAPI: blue Scale bar: 10 μm. (c) Quantification of confocal immunofluorescence images signals shown in B panel. (d) In situ PLA assay of SCAP-SREBP1 and SCAP-SREBP2 complex in Ctrl-1, Ctrl-2, GP73-1, and GP73-2 cells. SCAP-SREBP1 or SCAP-SREBP2 complex, red; nuclei, blue. (e,f) Quantification of PLA signals shown in D panel. Cell-based studies were performed at least three independent times with comparable results. Data represent mean ± SEM. Student’s t test was used for statistical analysis: **p < 0.01.
