Figure 6 | Scientific Reports

Figure 6

From: ATF3 negatively regulates cellular antiviral signaling and autophagy in the absence of type I interferons

Figure 6

ATF3 binds to the promoter region of Atg5. Neuro2a cells were mock-infected or infected with JEV (MOI 5) and at different time points chromatin immunoprecipitation was performed using ATF3-specific rabbit antibody or rabbit IgG (negative control for pull-down). Gene promoter-specific DNA was PCR amplified and separated on a 2% agarose gel. The relative abundance of pulled-down DNA during JEV infection as indicated by the PCR product is shown. The experiment was performed twice, and a representative figure is shown. Ten percent of total lysate was used as input.

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