Figure 2
From: Molecular hydrogen increases resilience to stress in mice
Repeated inhalation of hydrogen gas blocked the depressive- and anxiety-like behaviours in chronically stressed mice. (A) Experimental procedure. After a 5-day adaptation, mice were treated by chronic stress for 28 days. Beginning on day 14, mice inhaled mixture gas of H2/O2 [67%/33% (v/v)] or O2/N2 [33%/67% (v/v)] for 3 h daily or were injected with fluoxetine (10 mg/kg i.p.) daily 0.5 h before stress for 14 days. During day 28–30, behavioural tests were conducted to assess the depressive- and anxiety-like behaviours. Repeated inhalation of hydrogen gas significantly blocked the chronic stress-induced decrease in sucrose preference (B) without affecting the total intake (C) in the SPT, the increased latency to feeding (D) without affecting the total feeding in home cage (E) in the NSF test, and the decreased time spent in the central zone (F) without affecting the crossing activities (G) in the OFT. #P < 0.05 and ##P < 0.01 versus the control group; *P < 0.05 and **P < 0.01 versus the saline-treated CMS group. n = 8–10 per group. CMS, chronic mild stress; SPT, sucrose preference test; NSF, novelty suppressed feeding test; OFT, open field test.
