Figure 4

A reduction in cytosolic Ca²⁺ levels inhibits the proliferation and migration of human DASMCs. (a) Control (black) and test (red) groups are shown (n = 3). Normal DASMCs served as a control. DASMCs to which 10 µm KB-R7943 was added constituted the test group. (b) Quantification of mean calcium fluorescence intensity reveals that cytosolic calcium concentrations in DASMCs exposed to KB-R7943 are apparently reduced. (c) Calcium imaging showed that after added with 10 µm KB-R7943 the fluorescence in the DASMCs which represent for the cytosolic calcium concentration was reduced compared to the control group. The scale bar is 25μm. (d) Giemsa staining revealed a reduction in the number of transmembrane DASMCs following incubation with 10 µm KB-R7943. The scale bar is 100μm. (e) Numerical data for transmembrane DASMCs obtained following imageJ analysis (n = 5). (f) Microscopic photos demonstrating that KB-R7943-treated DASMCs grow slower than control groups. The scale bar is 200 μm. (g) Growth curves demonstrating a reduction in the growth of DASMCs following incubation with KB-R7943. The error bar indicate means ± SD. *P < 0.05, **P < 0.01.