Figure 4
Inhibition of MEF growth by MMC in SAM. (A) Phase contrast microscopy images of MEFs treated with 10 µg/ml MMC for 0–2.0 h in SAM and observed on days 1, 3, 5 and 7. Bars = 40 μm. (B) Cell counts of inactivated MEFs in each well of 24-well plate (Student’s t test and ANOVA; **P < 0.01 versus each group of days 1, 3, 5 and 7). (C) EdU-positive cells and representative images of fields show red-colored proliferation cells. Hoechst 33342 staining was performed to detect nuclear localization. Bars = 40 μm. (D) The number of EdU-positive cells were counted using ImageJ (Student’s t test; ***P < 0.001).
