Figure 9
Effects of VREF challenge and LysEF-P10 treatment on gut microbiota composition. The gut microbiota composition in the faeces of VREF-challenged and -unchallenged mice treated with or without LysEF-P10 was analysed using 16S rRNA gene sequencing (n = 6 mice per group). (A) Plot generated using the weighted version of UniFrac-based PCoA. (B) Bacterial taxonomic profiling at the phylum level of identified gut microbiota. (C) Phylogenetic distribution of microbial lineages in the faecal samples. (D) Abundance of gut microbiota at the genus level. (EF): mice challenged i.p. with 4 × 109 CFU/mouse of VREF E. faecalis E028; (EF.L): mice injected i.p. with 5 μg LysEF-P10 1 h after challenge with 4 × 109 CFU/mouse of E028; (L): mice injected i.p. with 100 μg LysEF-P10 only; (WT): mice treated with buffer, as a negative control.
