Table 7 Primer pairs of the Cx43 for sequence, real-time PCR and plasmid construction.
Primer Name | Sequence | Product Size (bp) |
|---|---|---|
Cx43-P1-Fwd | 5′-ATCTCCACCATTCCCTTTGTTA-3′ | 150 |
Cx43-P2-Rev | 5′-TGGGGAAGCATATAACCAGTTC-3′ | |
hCx43-Fwd | 5′-CAGCTTGTACCCAGGAGGAG-3′ | 209 |
hCx43-Rev | 5′TGTCCCTGGCCTTGAATATC-3′ | |
hGAPDH-Fwd | 5′-CACCAGGTGGTCTCCTCTGAC-3′ | 150 |
hGAPDH-Rev | 5′-GGTGGTCCAGGGGTCTTACTC-3′ | |
hB2M-Fwd | 5′-CGCTACTCTCTCTTTCTGG-3′ | 165 |
hB2M-Rev | 5′-TTCTCTCTCCATTCTTCAGTA-3′ | |
hCx43-inject-Fwd | 5′- GATCCTCGAG TAGTTTTCTGGAGACATGGTAGAGG-3′ | 500 |
hCx43-inject-Rev | 5′-GATCAGATCT TTACACTGCTTGCTTCACCAGA-3′ |
