Figure 6 | Scientific Reports

Figure 6

From: Cytoglobin Promotes Cardiac Progenitor Cell Survival against Oxidative Stress via the Upregulation of the NFκB/iNOS Signal Pathway and Nitric Oxide Production

Figure 6

Overexpressing CYGB increases the iNOS expression and nitric oxide production, which effect is abolished by iNOS inhibitor 1400 W. (a) Representative images and quantitative data of Western blot showing iNOS expression in hCPCs overexpressing CYGB vs. vector control. Full-length blots are presented in Supplemental Fig. S15. (b) Representative DAR-1 staining images and quantitative analysis for hCPCs infected with CYGB- or vector-lentivirus for 48 hours. (c) Representative images and quantitative analysis showing the traces for the measurement of nitric oxide levels by chemiluminescence in hCPCs overexpressing CYGB vs. vector control following the treatment with 100 µM 1400 W or vehicle. See experimental details in the Material and Methods Section. (d) Representative FACS analysis with DAR-1 staining showing the NO level in hCPCs overexpressing CYGB vs. vector control following the treatment with 100 µM 1400 W or vehicle. *Indicates p < 0.05 vs. control; **indicates p < 0.01 vs. control; n = 3 independent experiments.

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